Direct micromethod for colorimetry of serum ornithine carbamoyltransferase activity, with use of a linear standard curve.

A more sensitive automated method for determination of ornithine carbamoyltransferase activity in human serum.

Ornithine carbamoyltransferase (EC 2.1.3.3) activity is a sensitive, specific indicator of hepatocellular injury. This paper describes development of an improved automated procedure for measurement of this activity. Triethanolamine-ethylenediaminetetraacetate is used as a buffer, and activity is determined by measuring the concentration of the product, citrulline. Kinetic studies have been perf...

Phaseolotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: basis for immunity to phaseolotoxin.

Cell-free extracts from phaseolotoxin-producing strains of Pseudomonas syringae pv. phaseolicola grown at 18 degrees C, the optimum temperature for phaseolotoxin production, contain an ornithine carbamoyltransferase activity that is insensitive to phaseolotoxin. Extracts from the same strains grown at 30 degrees C, a temperature at which little or no detectable phaseolotoxin is produced, and fr...

Serum Ornithine Carbamoyltransferase Activity and Correlation with Fatty Liver in Dairy Cows with Displaced Abomasum

Corresponding Author: Ken Onda Azabu University, School of Veterinary Medicine, 17-71 Fuchinobe 1-Chome, Chuo-ku, Sagamihara 229-8501, Japan Tel: +81-42-754-7111 (ext. 658)18 Fax: +81-42-850-2464 Email: [email protected] Abstract: Ornithine Carbamoyltransferase (OCT) is a hepatocyte-specific enzyme; elevated serum activity indicates liver damage. Displaced abomasum frequently occurs around the...

Abnormal ornithine carbamoyltransferase in mice having the sparse-fur mutation.

Mice with the X-chromosomal sparse-fur (spf) mutation frequently have urinary bladder stones composed mostly of orotic acid, which was identified by the following criteria: ultraviolet and infrared absorption, spectra, chromatographic behavior, melting point, and reactivity in a specific color test. This clue led to the discovery that spf-bearing mice have an abnormal form of liver ornithine ca...

Significance of fixation in the cytochemical demonstration of ornithine carbamoyltransferase activity--with special reference to aldehyde perfusion.